Lenti-III-EF1α-hOSKM Virus

Lenti-III-EF1α-hOSKM Virus Description

The polycistronic lentivirus expresses all of the four human transcription factors (hOSKM of Yamanaka set: Oct4, Sox2, KLF4 or Myc) within a single expression cassette under the control of EF1α promoter. Each of the factor′s ORF is separated by a unique 2A peptide (P2A, T2A, and E2A) sequence, which allows for the equivalent expression of all four transcription factors from a single vector. The expression of all factors from a single virus minimizes the number of proviral integrations required for successful reprogramming. This also reduces the risk of insertional mutagenesis effects in the resultant induced pluripotent stem (iPS) cells when compared to those generated using four independent viruses. Also, the virus is provided as concentrated and VSV-G pseudotyped stock capable of transducing both dividing and non-dividing cells. A control GFP lentivirus is included for the easy tracking of gene transduction efficiency in your target cells.

The expression of these transcription factors has been shown to reprogram adult human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem cell (iPSCs).

Viral titer (cfu/mL) is determined by quantitative RT-PCR of viral stock preparation. Transcription factor protein expression are verified by immunocytochemistry and Western Blot analysis of transduced 293 cells. All our viral preparations are tested to be free of bacteria and other microbials.

Lenti-III-EF1α-hOSKM Virus Specification

Vector:	pLV-III-EF1a
Promoter:	EF1α
Genes:	Oct4, Sox2, KLF4 and Myc
Organism:	Human
Accession #:	Q01860, P48431, O43474 and P01106
Titer:	1x107 cfu/mL
Storage:	-70°C
Shipping:	On Dry Ice
References:	1)Takahashi et al. Cell. 2007 Nov 30;131(5):861-72. 2)Takahaski and Yamanaka. Cell. 2006 Aug 25;126(4):663-76.