Products
- FFPE, Frozen, and Viable Biospecimens and Biofluids with Clinical Annotation
- Viable Normal Biospecimens
- Liquid Biopsies
- Cell Immortalization Products
- Ready-to-Use Lentiviruses
- ALT assay
- Houdini™ Assay
- cDNA ORF Clones
- GPCR Cell Lines
- Phosphodiesterase Cell Lines
- Active Cell Extracts
- Circulating Nucelic Acids and Exosome Isolation
- Genomic DNA Isolation
- RNA Isolation kits
- Exosomes Isolation
- cfDNA Isolation Reagents
- Reporter Cell Lines
Polycistronic Viruses
The polycistronic lentiviruses can deliver to the target cell all four transcription factors within a single expression cassette under the control of EF1α promoter. Each ORF is separated by unique 2A peptide (P2A, T2A, and E2A) sequences allowing simultaneous expression of all four transcription factors from a single vector. Such approach minimizes the number of proviral integrations required for successful cell reprogramming. This also reduces the risk of insertional mutagenesis in the induced pluripotent stem (iPS) cells when compared to those generated using four independent viruses.
Vectors based on two protocols for both human and mouse sequences are available. Thomson's protocol (OSNL) includes Oct4, Sox2, Nanog or Lin28 genes, while Yamanaka's protocol (OSKM) includes Oct4, Sox2, KLF4 or Myc.
All viruses are VSV-G pseudotyped and capable of infecting both dividing and non-dividing cells. The expression of these transcription factors has been shown to reprogram adult human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem cell (iPSCs).