Viral RNA ISOLATION (Saliva, Viral transport MEDIum, etc.)
CBI offers a new system for the isolation of RNA, including viral RNA, from liquid biopsies, as well as from virus transport medium (VTM). Our RNA isolation protocol is a modification of the SubXTM cfDNA kit procedure. SubX molecules affinity to nucleic acids allows for immediate capture and preservation from degradation in bioliquid (saliva, serum, plasma, urine, VTM) without addition of inhibitors and/or chaotropic agents. No Proteinase K digestion is required. Standard volume of liquid biopsy for DNA isolation is 2-5 ml. Scale up to 50 ml of liquid can easily be done.
We have developed a new method of RNA isolation from liquid samples based on a dual-functional proprietary substance (SubX) that binds DNA and RNA under physiological conditions (e.g., directly from biological fluids) where it is absorbed to a solid phase Binding Matrix, e.g. magnetic beads.
Our RNA isolation procedure includes the following steps:
• RNA binding: simply add SubX and Binding Matrix to your bioliquid sample and rotate for a few minutes;
• Washing: 3 brief washing steps;
• Elution: RNA is easily eluted in a small volume (30-50ul) of buffer.
Figure 1. Amplification curves obtained for viral RNA isolated from serially diluted saliva samples spiked with lentivirus particles (Left panel). Plotting Ct values against viral RNA copy numbers (Right panel).
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