LIQUID BIOPSIES, EXOSOMES AND CFDNA ISOLATION
CBI offers prospective collection of plasma samples from patients, kits and reagents for efficient isolation of circulating exosomes and cell-free DNA (cfDNA) from liquid biopsies. Our SubX® cfDNA isolation kits have been specifically developed for efficient recovery of small DNA fragments. SubX®-Exo-DNA kit is designed for simultaneous isolation of exosomes and cfDNA directly from liquid biopsies (plasma or serum) without Proteinase K.
Introducing the SubX® Plasma cfDNA and SubX®-Exo-DNA Isolation Kits - Liquid Biopsy Made Easy
Unique features of SubX® that differ from all other technologies:
i - SubX® binds DNA under physiological conditions (e.g. directly in biological liquids) with high affinity degree, followed by adsorption of the [DNA-SubX®] complex on a solid phase matrix. SubX® captures DNA via its phosphate groups and allows for elimination of bias related to both AT/GC content and DNA fragments length, thus improving extraction efficacy and accuracy of downstream applications. Since our method does not require chaotropic salts for DNA capture, no dilution of starting material (and cfDNA) will take place.
ii - SubX® captures DNA in highly proteinaceous solutions without Proteinase K employment since it can dissociate or squeeze out histones and other DNA binding proteins from the DNA-Protein nucleosome complex.
iii - Both ends of SubX® molecule display phospholipid binding groups. This feature allows each molecule of SubX® to anchor two exosomes (i.e. dimerize and further oligomerize).
iv - SubX® oligomerizes exosomes in both highly proteinaceous bioliquids (serum, plasma) and in high salt biofluids (urine, tears, etc.). Excess of SubX® molecules in the solution results in oligomerization of up to 10-15 exosomes and formation of micron-size particles that are easily precipitated in a brief 14K x g centrifugation step. A specially designed buffer allows for reconstitution of the pelleted exosomes back to free monomer format suitable for downstream applications.
v – Different degrees of affinity of SubX® to DNA (strong) and to exosome phospholipid complexes (mild) allow to separate exosomes and cfDNA from the total pellet. Exosomes are easily recovered in monomer format by special reconstitute buffer while tightly bound [SubX®-cfDNA-beads] complex remains in pellet and cfDNA further isolated by supplied solutions.
Order Full cfDNA Kit (
List Price:$795/Discounted Price:$595)
Order Full Exo-DNA Kit (
List Price:$795/Discounted Price:$595)
The DNA-binding SubX® matrix allows:
• Flexibility. Scaling up of the procedure to 50 ml of starting material or scaling down to extra-low volumes as well as adaptation of the protocol for 96- or 384-well formats
• Efficiency. Separation of cfDNA from the bulk of proteins in a single vortex-spin step without employing chaotropic agents allows you to speed-up the isolation procedure to save time and overall costs
• Adaptability. Effectively bind cell-free DNA of virtually any size ranging from 100 Kbp down to 50 bp therefore extending downstream applications
Cell-free circulating DNA was discovered in 1948 but only recently has begun to attract the widespread attention of researchers. Historically, tumor tissue was considered as the major source of tumor DNA and thus invasive biopsy was the only way of obtaining such materials. Later, it was discovered that dying tumor cells release small pieces of their DNA into the bloodstream. These pieces are called cell-free circulating tumor DNA (ctDNA).
ctDNA has been detected in most types of cancer, even during early stages, suggesting the analysis of ctDNA at every stage of cancer may be used in personalized patient care. Thus, measurement of ctDNA levels in blood and identification of specific mutations in certain genes may also be used for estimation of cancer stage development and prognosis.