LENTIVIRUSES FOR CELL IMMORTALIZATION
We offer premade recombinant lentiviruses expressing SV40 large and small T antigens, Myc, Ras, Bmi1 and CDK4 genes, HPV16 E6/E7 protein, as well as siRNA against p53 and Rb.
Viral genes, including Simian virus 40 (SV40) T antigen, Epstein Barr virus (EBV), Adenovirus E1A and E1B, and human Papilomavirus (HPV) E6 and E7 can induce immortalization in different cell types. SV40 T antigen has been shown to be the most simple and reliable agent for the transformation of many different cell types in culture. The mechanism of SV40 T antigen in cell immortalization is well studied. For the most part, viral genes achieve immortalization by inactivating the tumor suppressor genes (p53, Rb and others) that can induce a replicative senescent state in cells. Recent studies have also shown that SV40 T antigen can induce Telomerase activity in the infected cells.