DNATraction^TM Multi-Purpose DNA Isolation Kit

Multi-purposes solid-phase DNA purification kit DNATraction^TM is intended for isolation of (i) genomic DNA from human, animal, and plant cultured cells or tissue samples; (ii) for DNA isolation from bacteria, phages and viruses; and (iii) for purification and concentration of amplified or labeled DNA fragments without employing of phenol/chloroform, DNAzol, proteinase K, alcohol precipitation and high speed centrifugation. The kit includes components sufficient for 50 standard DNA isolations from 1-10 mg of tissue or 0.5-2x10⁶ cells. During 30 min DNATraction^TM simultaneously generates ready-to-use high quality DNA from multiple samples suitable for amplification, restriction, ligation, labeling, sequencing, and cloning purposes. DNATraction^TM kit is applicable for general genetic engineering needs, genetic disease testing, viral and prenatal diagnostics, monitoring of HIV infection, forensic (HLA-Typing, paternity testing), and for genetic control of transgenic animals.

Advantages:

· Specially designed magnetic particles with ultra-low non-specific binding properties

· Fast: Ready-to-use DNA generated in half an hour.

· Convenient:  No ultracentrifugation, columns, cartridges, and alcohol precipitation.

· Safe:  No phenol/chloroform, SDS, and proteinase K

· Universal:  Extracts all forms of DNA: double-stranded, single-stranded, circular, and supercoiled plasmids, phage, bacterial, mitochondrial, plant, animal and human genomic DNA ranging from 50 bp to 200,000 bp.

· Versatile:  Simultaneous DNA extraction from cultured cells in 96-well format in the same cell culture plate.

Fig.1. Size distribution of genomic DNA isolated with DNATraction^TM kit from rat blood lymphocytes. Hexagonal pulse-field (CHIFE) gel electrophoresis in 1% agarose, 0.5xTBEM. EtBr staining. M - phage lambda DNA ladder; M’- yeast DNA ladder; Lane 1- DNA isolated by vortexing; Lane 2- DNA isolated by gentle pipetting. 

Fig. 2. Restriction endonucleases digestion of rat blood lymphocyte DNA isolated with DNATraction^TM kit. M-  1-kbp DNA ladder; Lane 1- digestion with EcoR I; Lane 2- digestion with BamH I; Lane 3- digestion with Hind III.

DNA isolation from cells cultured in 96-well plate. No extra plates, columns or tubes.  All procedure is done in the same plate where cells were grown!

Fig. 3. Magnetic beads before and after application to BioMag® 96-Well Plate Separator (Polysciences, Inc., Cat. 8MB4109S-1). 

Fig. 4. DNA yield per well from HEK293 cells grown in 96-well plate.