SIMULTANEOUS CFDNA AND EXOSOME ISOLATION FROM THE SAME SAMPLE (PLASMA, URINE)

cfDNA and Exosome Isolation can be done from the same sample!

The following diagram shows the sequence of steps involved in the process of separation and isolation of pure exosomes and cfDNA even from the small volume of the starting material (plasma or serum).

 

Figure 1.  Exosome and DNA isolation steps. All procedures after mixing plasma or urine with SubX® are done in one tube. Each incubation takes place at room temperature for 3-4 minutes with occasional vortexing. Both centrifugation or magnet separation can be used to remove solutions. Exosomes are separated from SubX®-DNA-Beads pellet by reconstitution in a special buffer ERB. DNA is eluted from the beads at 60°C.

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