The telomere repeat amplification protocol (T.R.A.P.) is the most widely used assay for telomerase activity. We have successfully developed our own real-time qPCR protocol in which we use a proprietary designed amplification primer that produces a fluorescent signal in the amplified product. In contrast to the published SYBR green-based real-time quantitative TRAPs, our approach provides attachment of only one fluorescent group to each amplicon produced in the assay thus allowing for accurate calibration of the assay by using a synthetic standard with telomeric repeats.