LENTIVIRUSES FOR CELL REPROGRAMMING
We offer premade Monocistronic and Polycistronic recombinant lentiviruses that can be used for the reprogramming differentiated cells into pluripotent state. The polycistronic lentiviruses can deliver to the target cell all four transcription factors within a single expression cassette under the control of EF1α promoter. Vectors based on two protocols for both human and mouse sequences are available. Thomson's protocol (OSNL) includes Oct4, Sox2, Nanog or Lin28 genes, while Yamanaka's protocol (OSKM) includes Oct4, Sox2, KLF4 or Myc.
Each ORF is separated by unique 2A peptide (P2A, T2A, and E2A) sequences allowing simultaneous expression of all four transcription factors from a single vector. Such approach minimizes the number of proviral integrations required for successful cell reprogramming. This also reduces the risk of insertional mutagenesis in the induced pluripotent stem (iPS) cells when compared to those generated using four independent viruses.
All viruses are VSV-G pseudotyped and capable of infecting both dividing and non-dividing cells. The expression of these transcription factors has been shown to reprogram adult human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem cell (iPSCs).
Standard stock's titer is about 107 IU/ml. High titer virus stocks are offered through custom lentivirus production service.
For safety issues related to recombinant lentiviruses please refer to the guidance documents available at the site of Office of Biotechnology Activities (NIH). Please click here.