ANALYSIS OF GENE EXPRESSION
Analysis of Gene Expression in Tissue Samples and Cell lines
We provide quantitative analyses of gene expression in tissue samples and cell lines by RT-qPCR. After isolation of total RNA from the samples, the specific RNA is reverse-transcribed, and the transcription of specific genes is measured either in a one-step or two-step RT-qPCR protocol.
Relative expression of various genes in a mouse spleen under specific conditions.