Human Cytokine Antibody Array 4, 120-plex Description

Analytes (10)
6Ckine, Axl, BTC, CCL28, CTACK, CXCL16, ENA-78, Eotaxin-3, GCP-2, GRO, HCC-1, HCC-4, IL-9, IL-17F, IL-18 BPa, IL-28A, IL-29, IL-31, IP-10, I-TAC, LIF, LIGHT, Lymphotactin, MCP-2, MCP-3, MCP-4, MDC, MIF, MIP-3alpha, MIP-3beta, MPIF-1, MSPalpha, NAP-2, OPN, PARC, PF4, SDF-1alpha, TARC, TECK, TSLP, AR, BDNF, bFGF, BMP-4, BMP-5, BMP-7, beta-NGF, EGF, EGF R, EG-VEGF, FGF-4, FGF-7, GDF-15, GDNF, GH, HB-EGF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-I, Insulin, MCSF R, NGF R, NT-3, NT-4, OPG, PDGF-AA, PIGF, SCF, SCF R, TGFalpha, TGFbeta1, TGFbeta3, VEGF, VEGF R2, VEGF R3, VEGF-D, BLC, Eotaxin, Eotaxin-2, G-CSF, GM-CSF, I-309, ICAM-1, IFNgamma, IL-1alpha, IL-1beta, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-6 sR, IL-7, IL-8, IL-10, IL-11, IL-12p40, IL-12p70, IL-13, IL-15, IL-16, IL-17, MCP-1, M-CSF, MIG, MIP-1alpha, MIP-1beta, MIP-1delta, PDGF-BB, RANTES, TIMP-1, TIMP-2, TNFalpha, TNFbeta, TNF sRI, TNF sRII

Format
One standard glass slide is spotted with 16 wells of identical cytokine antibody arrays. Each antibody is arrayed in quadruplicate.

Detection Method
Fluorescence with laser scanner: Alexa 555 (Cy3 wavelengths 555 nm excitation, 565 nm emission)

Sample Volume
Will be provided with the quote

Detection range
1- 3000 pg/ml

Standard curve range
5 - 800 pg/ml

Reproducibility
CV <20%

Sample Type
Conditioned medium, serum, plasma, urine, cell lysates