Human Cytokine Antibody Array 3, 60-plex Description

Analytes (10)
BLC, Eotaxin, Eotaxin-2, G-CSF, GM-CSF, I-309, ICAM-1, IFNgamma, IL-1alpha, IL-1beta, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-6 sR, IL-7, IL-8, IL-10, IL-11, IL-12p40, IL-12p70, IL-13, IL-15, IL-16, IL-17, MCP-1, M-CSF, MIG, MIP-1alpha, MIP-1beta, MIP-1delta, PDGF-BB, RANTES, TIMP-1, TIMP-2, TNFalpha, TNFbeta, TNF sRI, TNF sRII; AR, BDNF, bFGF, BMP-4, BMP-5, BMP-7, beta-NGF, EGF, EGF R, EG-VEGF, FGF-4, FGF-7, GDF-15, GDNF, GH, HB-EGF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-I, Insulin, MCSF R, NGF R, NT-3, NT-4, OPG, PDGF-AA, PIGF, SCF, SCF R, TGFalpha, TGFbeta1, TGFbeta3, VEGF, VEGF R2, VEGF R3, VEGF-D

Format
One standard glass slide is spotted with 16 wells of identical cytokine antibody arrays. Each antibody is arrayed in quadruplicate.

Detection Method
Fluorescence with laser scanner: Alexa 555 (Cy3 wavelengths 555 nm excitation, 565 nm emission)

Sample Volume
Will be provided with the quote

Detection range
1- 3000 pg/ml

Standard curve range
5 - 800 pg/ml

Reproducibility
CV <20%

Sample Type
Conditioned medium, serum, plasma, urine, cell lysates