Human Angiogenesis Array, 60-plex Description

Analytes (10)
Activin A, AgP, Angiogenin, ANG-2, ANGPTL4, bFGF, ENA-78, GRO, HB-EGF, HGF, IFNgamma, IGF-I, IL-1alpha, IL-2, IL-6, IL-8, IL-17, IP-10, Leptin, LIF, MCP-1, PDGF-BB, PIGF, RANTES, TGFbeta1, TIMP-1, TIMP-2, TNFalpha, TNFbeta, TPO, ANG-1, Angiostatin, CXCL16, EGF, FGF-4, Follistatin, G-CSF, GM-CSF, I-309, IL-1beta, IL-4, IL-10, IL-12p40, IL-12p70, I-TAC, MCP-2, MCP-3, MCP-4, MMP-1, MMP-9, PECAM-1, TGFalpha, TGFbeta3, Tie-1, Tie-2, uPAR, VEGF, VEGF R2, VEGF R3, VEGF-D

Format
One standard glass slide is spotted with 16 wells of identical cytokine antibody arrays. Each antibody is arrayed in quadruplicate.

Detection Method
Fluorescence with laser scanner: Alexa 555 (Cy3 wavelengths 555 nm excitation, 565 nm emission)

Sample Volume
Will be provided with the quote

Detection range
1- 3000 pg/ml

Standard curve range
5 - 800 pg/ml

Reproducibility
CV <20%

Sample Type
Conditioned medium, serum, plasma, urine, cell lysates