WNV Envelope Description
Shipped with Ice Packs
Introduction:
West Nile virus (WNV) is a virus of the family Flaviviridae part of the Japanese encephalitis (JE) antigenic complex of viruses. Image reconstructions and cryoelectron microscopyreveal a 45-50 nm virion covered with a relatively smooth proteinsurface. This structure is similar to virus; both belong to the genus flavivirus within the family Flaviviridae. WNV is a positive-sense, single strand of RNA, it is between 11,000 and 12,000 nucleotides long which encode seven non-structural proteins and three structural proteins. The RNA strand is held within a nucleocapsid formed from 12 kDaprotein blocks; the capsid is contained within a host-derived membrane altered by two viral glycoproteins.
Description:
The E.Coli derived 18kda recombinant protein contains the West-Nile N-terminus Envelope Virus immunodominant regions. The protein is fused with 6xHis tag.
Method:
Purified by proprietary chromatographic technique.
Purity:
Protein is >95% pure as determined by 10% PAGE (coomassie staining).
Formulation:
20mM phosphate buffer pH 7.5.
Storage:
Protein is shipped at ambient temperature.
Upon arrival, Store at -20°C.
Stability:
Five years frozen. One month in solution at room temperature.
Specificity:
Immunoreactive with sera of West Nile virus infected individuals.
Applications:
Antigen in ELISA and Western blots, excellent antigen for detection of West-Nile virus with minimal specificity problems.
Amino Acid Sequence:
MQLKGTTYGVCSKAFKFLGTPADTGHGTVVLELQYTGTDGPCKVPISSVA
SLNDLTPVGRLVTVNPFVSVATANAKVLIELEPPFGDSYIVVGRGEQQINH
HWHKSGSSIGKAFTTTLKGALE
Usage:
CBI's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
