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U2OS-Cyto
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U2OS-Cyto Description
Cytoplasm extract obtained from U2OS cells. Can be used for in vitro translation assays.
The human osteosarcoma U2OS cell line is one of the first generated cell lines and is used in various areas of biomedical research. U2OS is originally known as the 2T line, was cultivated from a moderately differentiated sarcoma of the bone tissue. Cell line U2OS is chromosomally highly altered, with chromosome counts in the hypertriploid range. Very few normal chromosomes are present, but a high number of stable marker chromosomes are identified. U2OS cells exhibit epithelial adherent morphology. Cells are positive for insulin-like growth factor I (IGF-I) and insulin-like growth factor II (IGF II) receptors, and express a number of antigens. The proteins of the U2OS cell line were analyzed using proteomics technology, and 237 different gene products were identified. The U2OS 2-D Electrophoresis database including 3000 individual spots analyzed provides the basis for future protein studies.