DyLightв„ў 488 Conjugated Affinity Purified anti-Mouse IgG (H&L) [Goat] Description
Product Name DyLightв„ў 488 Conjugated Affinity Purified anti-Mouse IgG (H&L) [Goat]
Size 100ug
Description Minimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins
Pictures 
Animal Mouse
Host Goat
Conjugation N-hydroxysuccinimide (NHS) ester
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Label DyLightв„ў 488 (MW 1,011.20); Green Emission
Absorption Wavelength 493 nm (in PBS)
Emission Wavelength 518 nm (in PBS)
Fluorochrome/Protein Ratio 4.0 DyLightв„ў 488 per mole of Goat IgG
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer 10 mg/ml BSA, IgG and Protease free
Preservative 0.01% (w/v) Sodium Azide
Applications This antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Instrument Compatibility The emission spectra for this DyLightв„ў conjugate match the principal output wavelengths of most common fluorescence instrumentation.
The emission spectra for this DyLightв„ў conjugate match the principal output wavelengths of most common fluorescence instrumentation. FLUORESCENT WESTERN BLOT >1:20,000
Storage Conditions This product is stable for several weeks at 4В° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20В° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of receipt if properly stored.
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of mouse IgG and with light chains of most mouse immunoglobulins.
Immunogen Mouse IgG, whole molecule
Notes This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin from Applied Biological Materials Inc. are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. DyLightв„ў is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.