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NIH/3T3-Nuc
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NIH/3T3-Nuc Description
Nuclear extract obtained from NIH/3T3 cells. Can be used for transcription and splicing in vitro assays.
Very widely used mouse fibroblast cell line, 3T3 cells have been derived from different mouse strains. NIH strain was established at the National Institute of Health in the USA from a Swiss mouse embryo. The spontaneously immortalized cells with stable growth rate were established after 20-30 generations. These cells are highly contact inhibited and are sensitive to sarcoma virus focus formation and leukemia virus propagation. The established NIH/3T3 line was subjected to more than 5 serial cycles of subcloning in order to develop a subclone with morphologic characteristics best suited for transformation assays. It is used for DNA transfection studies.