Products
- Human Tissue Repository
- Online Human Tissue Products
- Cell Immortalization
- Ready-to-Use Lentiviruses
- Viable Human Tissue
- siRNA, shRNA & miRNA
- Animal and Plant Tissue
- cDNA ORF Clones
- Viral Expression Systems
- Purified Proteins
- Stem Cell Research Tools
- Active Cell Extracts
- Proteomic Tools
- Houdini™ Assay
Services
- Telomere Maintenance Mechanism Assays
- Construction of siRNA-Lenti and siLenti-EGFP vectors
- Custom Lentivirus Construction
- Lentivirus Production
- Custom Avenovirus Construction
- Adenovirus Production
- Primary Cells Immortalization
- Stable Cell Line Production
- Multiplexed Protein Assay
- Protein Production
- miRNA Profiling
- Construction of miRNA Lentivirus Vectors
- Mouse Monoclonal Antibody Production
HeLa-Cyto
| Product Support |
|
|
HeLa-Cyto Description
Cytoplasm extract obtained from HeLa cells. Can be used for in vitro translation assays.
The HeLa cell line was derived for use in cancer research from a fatal cervical carcinoma. The HeLa genome was created by horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells. The genome is different from either parent genome including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. These cells proliferate abnormally rapidly, even compared to other cancer cells. HeLa cells have an active version of the enzyme telomerase during cell division, which prevents the incremental shortening of telomeres that is implicated in aging and eventual cell death. The cells allow for episomal amplification of transfected plasmids and extended temporal expression of the desired gene products.