Maltose Binding Protein Antibody

Maltose Binding Protein Antibody Description

Rabbit Polyclonal (IgG)

Immunogen: MBP epitope tag recombinant protein.

Specificity: Anti-MBP is optimally suited for monitoring the expression of MBP tagged fusion proteins. As such, anti- MBP/MBP can be used to identify fusion proteins containing the MBP epitope. The antibody recognizes the MBP epitope tag fused to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against MBP containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry, and other immunodetection techniques. Maltose binding protein is a bacterial protein, which is often used in protein expression studies because it creates a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest. It also allows for its easy purification from bacterial extracts under mild conditions. Anti-MBP is a companion to the pMAL protein expression system and can be used for the detection and purification of MBP-fusion proteins expressed in E. coli. By Western blot, a band is seen at ~ 42 kDa representing MBP.

Formulation: 1mg/ml in PBS, pH 7.4 with 0.05% sodium azide. Frozen liquid.

Storage/Stability: Stable for one year at –20?C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4?C for 3 months.

Application: Optimal working dilutions should be determined experimentally by investigators. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:1,000 Immunoprecipitation: 5 µg/mg lysate Histo/Cytochemistry: 1:200