Lenti-III-EF1α-mOSKM Virus

Lenti-III-EF1α-mOSKM Virus Description

The polycistronic lentivirus expresses all of the four mouse transcription factors (mOSKM of Yamanaka set: mOct4, mSox2, mKLF4 or mMyc) within a single expression cassette under the control of EF1α promoter. Each of the factor′s ORF is separated by a unique 2A peptide (P2A, T2A, and E2A) sequence, which allows for the equivalent expression of all four transcription factors from a single vector. The expression of all factors from a single virus minimizes the number of proviral integrations required for successful reprogramming. This also reduces the risk of insertional mutagenesis effects in the resultant induced pluripotent stem (iPS) cells when compared to those generated using four independent viruses. Also, the virus is provided as concentrated and VSV-G pseudotyped stock capable of transducing both dividing and non-dividing cells. A control GFP lentivirus is included for the easy tracking of gene transduction efficiency in your target cells.

The recombinant polycistronic lentivirus is provided as a VSV-G pseudotyped and concentrated virus stock capable of infecting both dividing and non-dividing cells. The expression of these transcription factors has been shown to reprogram adult mouse fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem cell (iPSCs).

Viral titer (cfu/mL) is determined by quantitative RT-PCR of viral stock preparation. Transcription factor protein expression are verified by immunocytochemistry and Western Blot analysis of transduced 293 cells. All our viral preparations are tested to be free of bacteria and other microbials.

Lenti-III-EF1α-mOSKM Virus Specification

Vector:	pLV-III-EF1a
Promoter:	EF1α
Genes:	mOct4, mSox2, mKLF4 and mMyc
Organism:	Mouse
Accession #:	P20263, P48432, Q60793 and P01108
Titer:	1x107 cfu/mL
Storage:	-70°C
Shipping:	On Dry Ice
References:	1)Yu et al. Science. 2007 Dec 21;318(5858):1917-20. 2) Yu et al. Science. 2009 May 8;324(5928):797-801. 3) Takahashi et al. Cell. 2007 Nov 30;131(5):861-72. 4)Takahaski and Yamanaka. Cell. 2006 Aug 25;126(4):663-76.