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HEK293-Nuc
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HEK293-Nuc Description
Nuclear extract obtained from human embryonic kidney (HEK) 293 cells. Can be used for transcription and splicing in vitro assays.
Human Embryonic Kidney (HEK) 293 cells were originally derived from human embryonic kidney grown in tissue culture. The cells allow for episomal replication of transfected plasmids and can be used for amplification of the plasmids and expression of the desired gene products. Since HEK cells express a number of adenoviral genes, they could be used to propagate adenoviral vectors in which these genes are deleted. HEK 293 cells have been widely used in cell biology research for many years and demonstrated to be a useful cell type to produce adenovirus, other viral vectors, and effectively glycosylated human recombinant proteins. They are also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy.