OmicsLink ORF Expression Clones (Full Length Protein Coding ORF Clones)

Description

Following the completion of the human genome project (HGP), elucidation of the functions of the proteins in the entire genome has become the rate-limiting step in the applications of the discoveries from HGP in biomedical research and pharmaceutical development. OmicsLink ORF expression clone collection offers more than 20,000 unique, validated and high quality full length protein coding cDNA clones (from start to stop codons) of human genes in various expression vector systems in ready-for-expression formats. OmicsLink clones have a widely range of applications in the areas of genomics, proteomics and other fields of biomedical and pharmaceutical research and development.

Ready for in vitro and in vivo Expression

Expression of proteins of your interest is now as easy as Click and Express by click-select-order of OmicsLink clones and expressing them immediately in your laboratory. OmicsLink now offers clones in several expression vectors with a variety of promoters, optimal translation signals (for example: Shine-Dalgarno and Kozak for E.coli and mammalian cell expression systems, respectively), and features, which makes these clones suitable for expression and functional studies in various cell systems. We also offer OmicsMate service to construct expression clones for other vector systems that are desired by customers but not yet included in our offered product line.

Application of OmicsLink Clones in Systems and Integrated Biology

  • Express proteins in E.coli cells
  • Express proteins in mammalian cells by reverse transfection
  • Produce proteins in in vitro cell-free translation systems
  • Express proteins with unique tag that facilitates purification and down-stream analysis
  • Generate probes of in situ hybridization for tissue or organ gene expression profiling

 

OmicsLink Clones v.s. Whole Transcript cDNA Clones

Clone Characteristics OmicsLink ORF clones Whole Transcript cDNA Clones
Gene transcript ORF (Start to stop only) Whole transcripts including 5' and 3' UTRs
Expression ready Yes Some are, most will need further subcloning procedures to remove 5' and 3' UTRs
Mammalian cell transfection    
Transicent Transfection Yes Only a small number
Stable Transfection Yes No
Unique protein tags Yes No
Protein-Molecule interactions Yes No
Concentration of lowly expressed proteins Yes No
Affinity purification of expressed proteins Yes No
Produce proteins via in vitro cell-free translation systems Yes No
Production Template for esiRNA Yes No
Probe generations for in situ hybridization Yes No
 

Quality Assurance

OmicsLink clones have been constructed from a collection of carefully curated and verified human full length cDNAs. The high quality of these clones is ensured by a stringent multiple-step QC/QA process strictly followed before every single clone is shipped to customers.

Expression Vector List
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