Products
Services
- Telomere Maintenance Mechanism Assays
- Construction of siRNA-Lenti and siLenti-EGFP vectors
- Custom Lentivirus Construction
- Lentivirus Production
- Custom Avenovirus Construction
- Adenovirus Production
- Primary Cells Immortalization
- Stable Cell Line Production
- Multiplexed Protein Assay
- Protein Production
- miRNA Profiling
- Construction of miRNA Lentivirus Vectors
- Mouse Monoclonal Antibody Production
Multi-Cistronic Vectors
Simultaneous delivery and expression of two or three genes by a single lentiviral vector is sometimes required. Most frequently IRES-based elements are used for these particular applications. However, it has been demonstrated that the genes inserted down-stream of IRES exhibit lower levels of expression. In addition, the IRES element is a relatively large fragment (> 800bp) and its use is often limited due to the lentiviral packaging capability (<5.0kb). Therefore a promoter system with bi-directional functionality has been developed for simultaneous expression of two or three different genes by a single lentiviral vector. Bi-cistronic vector is superior to IRES-based two-gene expression systems because of its smaller size (500bp) and stronger expression signal for both genes (Amendola et al., 2005). Up to 3 different genes can be expressed from the same lentiviral vector with the addition of a T2A peptide sequence (Tang et al., 2009).