Lentiviruses with Fluorescent and Bioluminescent Markers

CBI offers Ready-to-Use lentiviruses expressing eGFP, mCherry and mKate2 fluorescent proteins, as well as bioluminecstent marker Luciferase derived from two diffrent sources.

mKate2 is the next generation of monomeric far-red fluorescent protein TagFP635 (mKate) [Shcherbo et al., 2007; Shcherbo et al., 2009]. Possessing fluorescence with excitation/emission maxima at 588 and 633 nm, mKate2 is almost 3-fold brighter than TagFP635 and is 10-fold brighter than mPlum at the physiological pH = 7.5. Within the optical window optimal for light penetration in living tissues, calculated brightness of mKate2 is at least 2-fold higher compared to any monomeric fluorescent protein reported to date. 

Lentiviral vector with mKate2 is ideally suited for transduction of mammalian cells that can be implanted into animals for in vivo imaging.