Products
Services
- Telomere Maintenance Mechanism Assays
- Construction of siRNA-Lenti and siLenti-EGFP vectors
- Custom Lentivirus Construction
- Lentivirus Production
- Custom Avenovirus Construction
- Adenovirus Production
- Primary Cells Immortalization
- Stable Cell Line Production
- Multiplexed Protein Assay
- Protein Production
- miRNA Profiling
- Construction of miRNA Lentivirus Vectors
- Mouse Monoclonal Antibody Production
i-Lenti RNAi Expression System
The iLenti siRNA vector represents a third generation of lentiviral vectors derived from HIV‐1 based lentivirus. The iLenti siRNA vector is designed with convergent Pol III promoters that drive the transcription of target siRNA from opposite directions. The subcloning of target siRNA is achieved by a simple ligation of annealed target siRNA to the Bbs I linearized iLenti vector. The linearized iLenti vector contains two different 5′ overhangs to reduce the occurrence of background self‐ligation and mis‐directional cloning.