Construction of siRNA-Lenti and siLenti-EGFP vectors

Advantages of siRNA-pLV Constructs:

• RNAi in lentiviral vectors can be used for transfection or viral infection.
• siRNA-pLV vectors are very stable and can be amplified in regular competent cells.
• Lentiviral vectors offer the highest, stable gene transduction efficiency.
• Choice of antibiotic resistance markers: Neo or Puro 
• siRNA-pLV uses 27-29 bp oligos, which are more efficient than traditional 21mers.
• siRNA-pLV uses convergent promoters to avoid hairpin loop structures, making sequencing and plasmid growth much easier.
• siRNA-pLV saves money on oligos, as ~35 instead of 70bp oligos are used for the expression of double standed RNAi in vivo.

siRNA-pLV Map	siRNA-pLV-EGFP Map