Primary Cell Immortalization Service

We are offering service for the immortalization of primary cells. We employ the most comprehensive cell immortalization products that comprise of recombinant retroviruses and adenoviruses expressing Myc, p53, Rb, Ras, and SV40 T antigens. All these tools make our cell immortalization process simpler and easier than ever before. We can significantly extend replicative capacity of your target cells.

Primary mammalian cells reach senescence after a certain number of population doublings, and that is a limiting factor for cell culture maintenance. Re-establishing fresh cultures from explanted tissue is a tedious process. Immortalized cells have extended replicative capacity and can be used consistently throughout the entire research project.

Immortalization methods include transduction of mammalian cells with vectors bearing viral genes, such as Simian virus 40 (SV40) T antigen, Epstein Barr virus (EBV), Adenovirus E1A and E1B, and human Papilomavirus (HPV) E6 and E7. SV40 T antigen has been shown to be the most simple and reliable agent for the transformation of many different cell types in culture. The mechanism of SV40 T antigen in this process is well studied. Immortalization is achieved by inactivating the tumor suppressor genes (p53, Rb and others) with viral genes and that can induce a replicative senescent state in cells. Studies have shown that SV40 T antigen also induced telomerase activity in the infected cells.

We work with cells from the following tissues: Brain • Liver • Lung • Ovarian • Pancreas • Prostate • Skin • Thoracic aorta • Umbilical Cord

• Different systems can be tested
• Guaranteed >95% of cell population containing stably integrated gene that induces immortalization
• Average turnaround time is 2-3 months, although it depends on the cell type
• We also can provide primary cells

Please submit the Request Form for this service and we will send you a detailed quotation within 24 hours.