Products
Services
- Telomere Maintenance Mechanism Assays
- Construction of siRNA-Lenti and siLenti-EGFP vectors
- Custom Lentivirus Construction
- Lentivirus Production
- Custom Avenovirus Construction
- Adenovirus Production
- Primary Cells Immortalization
- Stable Cell Line Production
- Multiplexed Protein Assay
- Protein Production
- miRNA Profiling
- Construction of miRNA Lentivirus Vectors
- Mouse Monoclonal Antibody Production
Polycistronic Viruses
The polycistronic lentiviruses can deliver to the target cell all four transcription factors within a single expression cassette under the control of EF1α promoter. Each ORF is separated by unique 2A peptide (P2A, T2A, and E2A) sequences allowing simultaneous expression of all four transcription factors from a single vector. Such approach minimizes the number of proviral integrations required for successful cell reprogramming. This also reduces the risk of insertional mutagenesis in the induced pluripotent stem (iPS) cells when compared to those generated using four independent viruses.
Vectors based on two protocols for both human and mouse sequences are available. Thomson's protocol (OSNL) includes Oct4, Sox2, Nanog or Lin28 genes, while Yamanaka's protocol (OSKM) includes Oct4, Sox2, KLF4 or Myc.
All viruses are VSV-G pseudotyped and capable of infecting both dividing and non-dividing cells. The expression of these transcription factors has been shown to reprogram adult human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem cell (iPSCs).